選擇Y subcoclumns for replicates or error bars 下的第三個enter and plot error values already calculated elsewhere ,enter中選擇mean,SD,N即可
1. 首先把你的數(shù)據(jù)在Excel 中按以下方式排列: 第一列, 藥物濃度要換算成log值, 第二列, 與第一列對應(yīng)的pecent inhibiton, 第三列為 對應(yīng)的error bar.文章源自四五設(shè)計(jì)網(wǎng)-http://www.wasochina.com/51775.html
2. 打開Graphpad, 選擇Y選項(xiàng)時選擇 ENTER: Mean&SD,進(jìn)去后COPY 你的數(shù)據(jù)進(jìn)去,按照下面步驟進(jìn)行 Analyze---進(jìn)入XY analyses 選擇Nonlinear regression一直O(jiān)K下去,到Choose an equation中選Dose-response -Inhibition里面的log(inhibitor) vs response---Variable slope就好了.文章源自四五設(shè)計(jì)網(wǎng)-http://www.wasochina.com/51775.html
當(dāng)然,有時你的點(diǎn)趨勢不好時,你還得限制下Top or Bottom or both of them.文章源自四五設(shè)計(jì)網(wǎng)-http://www.wasochina.com/51775.html
同樣,EC50也差不多這樣,只是后來選擇的是Dose-response -Stimulation.文章源自四五設(shè)計(jì)網(wǎng)-http://www.wasochina.com/51775.html 文章源自四五設(shè)計(jì)網(wǎng)-http://www.wasochina.com/51775.html
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